Journal: Molecular Metabolism
Article Title: Roles for Prlhr /GPR10 and Npffr2 /GPR74 in feeding responses to PrRP
doi: 10.1016/j.molmet.2024.102093
Figure Lengend Snippet: p52, but not NTS PrlhOX or the activation of NTS Prlh neurons, suppresses locomotor activity in mice . A. Locomotor activity in wild-type mice treated with vehicle and p52 (n = 12, 5 mg/kg IP), respectively. B. Locomotor activity in Prlh Cre (n = 6; Ctrl-WT) and NTS PrlhOX (n = 7, PrlhOX-WT) mice. C. Locomotor activity in control mice (Ctrl; n = 9) and NTS Prlh−Dq mice (Dq; n = 3) treated with either saline or CNO (1 mg/kg IP) twice daily. All graphs: Shown is mean beam breaks per hour ± SEM for the light cycle, the dark cycle, and averaged over the entire 24-hour day. Multiple paired t-tests, Holm-Šídák method and Šídák-Bonferroni method were used; p values are shown for significant comparisons. ∗: p < 0.05, ∗∗: p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗: p < 0.0001.
Article Snippet: The long-acting PrRP analog p52 (Palm-SRTHR HS-Nle-EI RTPDI NPAWY ASRGI RPVGR Phe(pNO2)-amide) was synthesized on a Prelude (Gyros Protein Technologies) by automated synthesis using the Fmoc/ t Bu protocol, purified by reversed-phase HPLC and characterized by single quadrupolar LC/MS using a Waters Mass Lynx 3100 platform.
Techniques: Activation Assay, Activity Assay, Control, Saline